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Information × Registration Number 0213U003082, 0110U002197 , R & D reports Title Obtaining and functional characteristics study of umbilical cord mesenchymal stem cells and DNA-vectors as theraupetic instruments for cartilage and vessels pathologies usage popup.stage_title Head Kordium Vitalii Arnoldovych, Registration Date 18-01-2013 Organization Institute of genetic and regenerative medicine Acad.Med.Sci. of Ukraine popup.description2 Technical protocol for MSC isolation from umbilical cord has been developed, their multiplication and differentiation in vitro were worked out. The protocol takes into account sample individual properties. MSC characteristics (morphology, surface markers expression) were studied in connection with cultivation parameters and amount of passages. Recombinant plasmid containing human apolipoprotein A1 gene was created and effective in vitro and in vivo. non-viral genes transfer system was developed. 3 cell lines of stable transformants expressing human apolipoprotein A1 were obtained. The model of streptozotocin-induced hyperglycemia combined with cholesterol dislipidemia was developed in rabbits. Minimal dose of recombinant DNA containing human apolipoprotein A1 gene, direct intraliver injection of which (under ultrasound control) leads to compensation of typical for atherosclerosis lesions of tissues and vessels in model animals has been determined.. Product Description popup.authors Горбатюк О.Б. Дерябіна О.Г. Ковальчук М.В. Маслова О.О. Николаєв Ю.С. Павлова М.В. Похоленко Я.О. Римар С.Ю. Топорова О.К. Шувалова Н.С. popup.nrat_date 2020-04-02 Close