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Information × Registration Number 0213U003547, 0110U005785 , R & D reports Title Metabolic engeneering of lipoglikopeptides antibiotic teicoplanin by Actinoplanes teighomyceticus popup.stage_title Head Shulga Sergiy Michaylovich, Кандидат фізико-математичних наук Registration Date 07-02-2013 Organization Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine popup.description2 Constructed recombinant kosmidy for gene knockouts tcp28 and tcp29 by rekombinirynhu based kosmidy 19FG. 8 constructed plasmids and overexpression of genes tcp28 tcp29 based integrative vector pSET152 and replicative vectors rKS1218E (1 copy) or rKS1139 (30-40 copies per genome). All designs tested by PCR or sequencing. All constructs transferred into cells A. teichomyceticus, set output level teicoplanin obtained transkon'yuhantamy in liquid canola environment. Gene overexpression tcp28 significantly increased the level of production of teicoplanin. Product Description popup.authors Андріяш Г.С. Бейко Н.Є. Заболотна Г.М. Осташ Б. Ткаченко А.Ф. Федоренко В. Хоменко А.І. Шульга С.М. popup.nrat_date 2020-04-02 Close