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Information × Registration Number 0213U003549, 0112U003496 , R & D reports Title Isolation of small regulatory RNA preparations from cells of plants infected by pathogens and treated by growth regulators, their identification and testing their biological activity in cell-free systems of protein synthesis. popup.stage_title Head Galkin Anatoliy Pavlovich, Registration Date 07-02-2013 Organization Institute of Food Biotechnology and Cenomics of the National Academy of Sciences of Ukraine popup.description2 . New complex plant growth regulators with bioprotective activity are created together with Institute of Microbiology and Virology NAS of Ukraine: Averkom (producer - Streptomyces avermitilis UKM Ас-2179) and its modifications. In field and greenhouse experiments at the wheat, tomatoes, potato and cucumbers plants, treated by these regulators, the considerable decrease in their defeat by parasitic phytonematodes Meloidogyne incognita and Diethylenchus Destructa, as well as increase of productivity of these cultures in the conditions of an infectious background are established: at cucumbers - on 22-26 %, at tomatoes - on 16-62 %, wheat - on 2-16,9 % and a potato - 9,3-12,0 %. By molecular-biological methods in cell-free systems of protein synthesis from wheat sprouts the high level (38-65 %) is confirmed of antinematode silencing activity of small regulatory si/miRNA, isolated from cells of infected by nematodes and treated by complex preparations plants. By DOT-blotting method the considerable difference (6-23 %) is established between populations of si/miRNA and cytoplasm mRNA isolated from cells of treated by complex preparations and control plants. Product Description popup.authors Іутинська Г.О. Білявська Л.О. Галкін А.П. Галкіна Л.О. Козирицька В.С. Циганкова В.А. popup.nrat_date 2020-04-02 Close