Search academic texts

Information × Registration Number 0213U004871, 0112U006840 , R & D reports Title Translation aparatus in cytosol: their role in mitochondrial translation and antioxidant homeostasis popup.stage_title Head Negrutskii B.S, Registration Date 26-12-2013 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 Oncogene PTI- 1 was identified in prostate cancer cell culture and human invasive prostate adenocarcinoma , but not in healthy tissue . A DNA segment encoding this protein is similar with the part encoding elongation translation factor human eEF1A1. Oncogenic mechanisms of PTI -1 is unknown. It is unclear what, RNA or protein product have such activity. The protein product of the gene PTI -1 is obtained by overexpression in mammalian cells. As a model of RTI -1 was used eEF1A, were by limited proteolysis was removed 69 amino acids from the N-terminus. This construction is very similar to the protein PTI -1 and is named ?eEF1A. The objectives of our study was obtaining sufficient amount of eEF1A from rabbit liver , a quantitative obtaining of eEF1A by limited proteolysis, and the study of the question - whether lose some of the nucleotide exchange factors ability to interact with ?eEF1A, that may be one of the mechanisms affecting to the function PTI -1 in reducing the accuracy of translation in tumor cells Product Description popup.authors Висловух А.А Власенко Д.О Новосильна О.В Порубльова Л.В Тросюк Т.В. Шалак В.Ф. popup.nrat_date 2020-04-02 Close