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Information × Registration Number 0213U004894, 0110U005553 , R & D reports Title Complex project: Interaction between nanoscale materials with normal and tumor cells, development of methods of their delivery safety of their use. popup.stage_title Head Chekhun Vasyl Fedorovych, Registration Date 27-12-2013 Organization R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology popup.description2 We studied impact of nanomaterials of different origin (fullerenes, nanotubes, gold nanoparticles, magnetic fluid) on phenotypic and cytogenetic features of normal and tumor cells (human and animal breast cancer cells with different morphological and growth characteristics, rat fibroblasts). We found that studied nanomaterials caused differen effects, which in part depend on the origin of the factor, but in most part - on its concentration and histogenetical properties of cells. We showed that all studied nanomaterials in low concentrations stimulated proliferative effects in cells of mesenchymal origin. At the same time, high conxentrations of nanomaterials, particularly, magnetic fluid stopped proliferation of cells of epithelial origin. According to the results of cytogenetic studies, breast cancer cells with mesenchymal phenotype were most sensitive to the impact of nanoparticles. We also studied impact of nanomaterials of different origin on nitric oxide and free radicals production by peritoneal macrophages. We showed that nanotubes caused elevation of number and functional activity of peritoneal macrophages, what is the marker of activation of NO-synthase activity in these cells. Nanoferromagnetic and gold nanoparticles used in studied concentrations did not cause signafacnt changes on synthesis of free radicals by peritoneal macrophages. By means of IR spectroscopy, circle dichroism, tryptophan fluorescence and differential scanning microcalorimetry we found that interaction of gold nanoparticles, carbon nanotubes, fullerenes and magnetite with serum albumin from healthy donors caused some changes in secondary and tertiary structure of protein globule. This finding is confirmed by data about reduction of number of natural ?-helixes, appearance of additional ?-structures, hydrophobic composition of serum albumin fluorophore and changes of its thermostability. Using desolvatation method we obtained stable solutions of nanocomposites based on albumin nanoparticles and nanomaterials of different origin for further study on of their action on main features of organs and systems of experimental animals in normal state and during tumor growth. We performed series of studies aimed at determination of toxicity of differently shaped gold nanoparticles and other nanomaterials for normal and transformed human lymphocytes. We showed that neither free nor conjugated with folic acid gold nanoprisms show significant own (dark) toxicity on МТ-4 and Namalwa cells, as well as on normal and malignized human lymphocytes from healthy donors, or from B-CLL patients. Spherical gold nanoparticles of small diameter (5 nm) showed some toxic effects on both lymphoid cultures. Fullerenes and carbon nanotubes did not cause marked impact on cell death. Magnetic fluid stimulated cell growth. All nanoparticles in the form of albumin composites were not toxic against lymphoid cultures in concentrations equal to maximum permissible doses. Product Description popup.authors Бездєнєжних Наталія Олександрівна Гамалея Микола Федорович Главін Олексій Анатолійович Ковальова Оксана Анатолівна Кудрявець Юрій Йосипович Лихова Олександра Олександрівна Лук'янова Наталія Юріївна Мельник Наталія Миколаївна Михайленко Віктор Михайлович Сарнацька Вероніка Вячеславівна Семесюк Надія Іванівна Тодор Ігор Миколайович Чехун Василь Федорович Швець Юлія Вікторівна Штонь Ірина Олександрівна Юшко Лариса Олексіївна popup.nrat_date 2020-04-02 Close