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Information × Registration Number 0213U005336, 0111U000799 , R & D reports Title To study the immunobiological properties of the recombinant and culture antigens of BLV to improve its diagnostics popup.stage_title Head Gorbatenko Stanislav Kondratevich, Registration Date 10-04-2013 Organization Nationali Scientific Center "Institute of Experimental and Clinical Veterinary Medicine" popup.description2 The bioinformative analysis of BLV genes for recombinant antigens obtaining was carried out. The protocol of pUc19_bt_colprom2 expressing plasmid synthesis was worked out. The E. coli HB101 clone transformed with sequence coding BLV gp51 and p24 regions was obtained. . The cultural BLV antigens with increased activity were obtained using the modified nutrient media. The maximum specific activity in the ELISA had the antigen with the 1.136 g/cm3 density, obtained by ultracentrifugation using a nonidet detergent. The antibovine immunoglobulin, immunoperoxidase conjugate, ovine specific IgG, negative and positive reference serum, bovine serum globulins, as components of ELISA and ICA, were manufactured. Product Description popup.authors Бриль Ніна Федорівна Горбатенко Станіслав Кіндратович Долгіх Людмила Геронтіївна Дуднік Вера Олександрівна Зданєвич Петро Петрович Корнєйков Олександ Миколайович Кузнецова Олена Валеріївна Мягких Ніна Васильєвна Чугунова Наталія Володимирівна Шаповалова Ольга Вікторівна popup.nrat_date 2020-04-02 Close