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Information × Registration Number 0213U005580, 0112U005549 , R & D reports Title "Investigation of influence metal and carbon nanomaterials as matrix for differentiation growing cells in vitro" popup.stage_title Head Sydorenko Mychail V., Registration Date 19-07-2013 Organization Department of Biotechnical Problems of Diagnostics, Institute of Problems of Cryobiology and Cryomedicine of NAS of Ukraine popup.description2 Report on Phase III research on "Evaluation kantseroprotektiv qualities of iron and carbon nanostructures using a model of multicellular spheroids" research on "Investigation of the influence of iron and carbon nanomaterials as prodiferentsiynogo matrix for growing cells in vitro". 59 p., 12 fig., 4 photos, Table 5, 68 sources of references. The object of study - ultra-nanodiamond, nanotubes and fullerenes, tumor cell lines of adenocarcinoma of the breast (MCF-7). The goal - a comparison of the qualities of carbon nanomaterials (diamond-like carbon nanotubes, fullerenes) as an adhesive matrix for growing cells with a transformed phenotype and study the features of cell morphogenesis in a modification of nanostructures. Methods of research - the methods of cell culture, cyto-morphological, MTT test imunotsitochimichemistry staining methods of spectral studies of Fourier spectrometry. The study revealed the following: the size and structure of carbon units determines the possible effect of the UDD and HT on the growth, proliferation and adhesion of cells in culture determines the further development of the cell population. UDD units ranging from 10-4 to 10-2 mkm3 (50-220 nm) at a concentration of 12.5 to 50 mcg/ml causing a reduction in focal cell substratum contacts, which reduces the adhesion of the cells, and reduce the number of small cell spheroids. Increasing the concentration of UDD not affect the effectiveness of generating cell spheroids. Nanotubes at the same concentrations constitute self-assembled aggregates of 0.2-10 mkm3 (500 nm-2,2 microns), which can act as an artificial extracellular matrix. Keywords: tumor cells of breast cancer, ultra nanodiamonds, multicellular spheroids. Product Description popup.authors Бурлака Дмитро Петрович Перепелиціна Олена Михайлівна Ястребова Олена Вікторівна popup.nrat_date 2020-04-02 Close