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Information × Registration Number 0213U005587, 0109U004189 , R & D reports Title The role of calcium store in regulation of short-time synaptic plasticity in inhibitory spinal cord interneurons. popup.stage_title Head Veselovskiy Nikolay S., Registration Date 23-07-2013 Organization International center of molecular physiology popup.description2 Primary afferent (DRG) neurons and the dorsal horn spinal cord (DH) neurons play a fundamental role in transmission and modulation of different types of somatic and visceral signalization. We examined the features of induction of use-dependant short- and long-term depression (LTD) of glutamatergic and glycinergic synaptic transmission in DH neurons that was evoked by low- frequency (LFS) 5 Hz conditioning stimulation of DRG neurons. Using dual whole-cell patch clamp recording on the pre- and postsynaptic cells simultaneously we analyzed the excitatory and inhibitory postsynaptic currents from DH neurons, evoked by the intracellular stimulation of DRG neurons, in pairs of co-cultivated cells. In co-culture of DRG-DH neurons prolonged conditioning stimulation of DRG cell (5 Hz) induced LTD of AMPA-activated eEPSCs amplitudes in DH neurons to 57,33 ± 2,5 % of control at least 20 minutes. We found out that the LTD magnitude increased with the number of low-frequency conditioning pulses. Our data suggest a possibility of induction of activity-dependant homosynaptic LTD of AMPA-receptor mediated glutamatergic neurotransmission at the level of pair synaptically connected DRG and DH neurons in co-culture. Such LFS induced LTD is dependant on timing activation of presynaptic DRG neuron and not requires of postsynaptic DH cell depolarization. Product Description popup.authors Кошелева С.О. Рихальський О.В. Шипшина М.С. popup.nrat_date 2020-04-02 Close