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Registration Number

0213U006563, 0111U006621 , R & D reports

Title

Structural rearrabgements of components of the cardiovascular system in its normal and abnormal histogenesis in humans and experimental animals

Head

Tverdokhlib Igor,

Registration Date

20-11-2013

Organization

State organization "Dnepropetrovsk medikal akademy of Ministry Health Ukraine"

Description

The object of study - histogenesis of conduction system, epicardium, outflow tract, cardiac chambers, development and ultrastructural reorganization of intercalated disks of contractile cardiomyocytes and mitochondria. Purpose - to establish the quantitative and qualitative characteristics of histogenetic rearrangements of the conducting system of the heart, epicardium, outflow tract, histostructural and ultrastructural changes of the heart chambers, formation of intercalated disc and mitochondria of contractile cardiomyocytes at stages of ontogenegenesis in norm and under the experimental conditions. Methods: histological, immunohistochemical, electon microscopy, three-dimensional computer modeling, termination of liver bud development, dehydration of maternal organism and prenatal hypoxia, morphometric, biometric. Histogenetic rearrangements of the conducting system of the human heart during prenatal ontogenesis was investigated; the term of the beginning of definitive cardiac conduction system links formation was defined; the time of the establishment of connections between them and the terms of registration of the conduction system as an integrated structure; aspects of the specificity of the expression of antigenic and carbohydrate determinants of conductive cardiomyocytes during different periods of cardiogenesis were studied. The work identifies the main histogenetical processes underlying the formation of the epicardium and coronary endothelial in human embryos, and the impact of interruptions in the development of the liver on the early stages of prenatal ontogenesis on the formation of the epicardium and the coronary vessels of chicken embryos. A quantitative assessment of the qualitative morphological transformations of the outflow region of mouse heart in the dehydration of the maternal organism were defined. Histogenetical and ultrastructural characteristics of the heart chambers of human and chicken embryo in systole and diastole were performed. Quantitative and qualitative characteristics of intercalated disks of contractile rat cardiomyocytes during ontogeny was established. The basic mitochondrial ultrastructural changes of the contractile cardiomyocytes of rat under conditions of chronic prenatal hypoxia were studied. The results of research are integrated into scientific process of 11 higher educational institutions of Ukraine. Based on the results of the study 1 patent of Ukraine for useful model was obtained. Key words: myocardium, conduction system, the epicardium, embryonic heart, outflow tract of the heart, maternal dehydration, intercalated disk, mitochondria, prenatal hypoxia.

Product Description

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Іванченко М.В.
Гудлетт Т.О.
Дяговець К. І.
Марченко Д.Г.
Петрук Н.С.
Потоцька О.Ю.
Сілкіна Ю.В.
Твердохліб І.В.
Шевченко К.М.