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Information × Registration Number 0213U006718, 0113U004306 , R & D reports Title Substrate specificity and topological features of the active site of cytochromes P450 7A1, 7B1 and 2E1 popup.stage_title Head Chashchyn Mykola Oleksiyovych, Registration Date 25-12-2013 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 The aim is the construction of a heterologous system for the expression of recombinant human cytochrome P450 2E1 and conducting site-directed mutagenesis of the nucleotide sequence of its gene. During the project the functional bicistronic plasmid construct pCW/2E1/OR which provides simultaneous expression in E.coli cells by cytochrome P450 2E1 and NADPH-P450 reductase was obtained. Also site-directed mutagenesis of the cDNA nucleotide sequence of the gene of human cytochrome P450 2E1 was carried out and the plasmid constructs pCW2E1-106, pCW2E1-212 and pCW2E1-298 for the expression of recombinant human cytochrome P450 2E1, that carry a mutation in the active center of the enzyme, was obtained. Product Description popup.authors Заєць Володимир Миколайович Кітам Володимир Олегович Максимчук Оксана Василівна Росохацька Інна Василівна Рущак Володимир Володимирович popup.nrat_date 2020-04-02 Close