Search academic texts

Information × Registration Number 0213U006719, 0113U007553 , R & D reports Title Development of biosensor test systems and approaches to improving their analytical characteristics popup.stage_title Head Soldatkin Oleksandr Oleksiyovich, Registration Date 25-12-2013 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 The aim of the project was to develop biosensors with sensitive membranes based on immobilized enzymes and evaluate their characteristics. Analytical characteristics of the biosensor based on glucose oxidase and hexokinase and intended for ATP determination were studied. Platinum disc electrodes were used as amperometric transducers. Range of working potentials for biosensor functioning was shown. An optimal time of enzymes immobilization was determined. Optimal conditions for biosensor functioning during work with biological fluids were selected. I.e., biosensor work in three buffer solutions (PBS, tris and HEPES) was investigated and it was shown that it is possible to obtain various operational characteristics of the biosensor depending on tasks that are assigned to it by varying the composition of sample. Reproducibility of biosensor responses to ATP and glucose during a day and reproducibility of biosensor preparation were shown. The proposed biosensor can be further used for analysis of glucose and ATP content in water solutions. Product Description popup.authors Архипова В.М. Костюченко Н.І. Кучеренко І.С. Пушкарьов В.М. Солдаткін О.О. Степурська К.В. popup.nrat_date 2020-04-02 Close