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Information × Registration Number 0213U006776, 0110U006124 , R & D reports Title Developing a fundamental basis for obtaining new generation immunoreagents popup.stage_title Head Okunev Oleg Volodymyrovych, Registration Date 30-12-2013 Organization Institute of Molecular Biology and Genetics popup.description2 Specificity of obtained in the previous stages of investigation from the immune combinatorial libraries of recombinant single-chain antibody against CD34 was confirmed in a Western blot with lysates CD34-positive cells KG1. The DNA sequences encoding S. aureus protein A (SPA) and bacterial alkaline phosphatase with enhanced catalytic activity (BAPmut) were used for construction of gene encoding fusion protein SPA-BAPmut that was expressed in the high-productive E. coli system and obtained in a soluble form. Cultivation conditions to provide a high-level expression of SPA-BAPmut (> 1 g/l) were determined. The target protein was obtained with purity more than 95 % using IMAX method. The possibility of application of SPA-BAPmut as sensitive and universal secondary immunoreagent for diagnostic tests was shown.5481 Product Description popup.authors Gorbatiuk O. B. Okunev O. V. Shpileva S. P. popup.nrat_date 2020-04-02 Close