Search academic texts

Information × Registration Number 0213U006790, 0113U000460 , R & D reports Title Methodological approaches elaboration to the matter of biological preservation of tissue typing of cord blood samples providing as a basis for the creation of donor hematopoietic tissue cryobanks popup.stage_title Head Kalynychenko T.O., Доктор медичних наук Registration Date 30-12-2013 Organization Kiev Research Institute of Haematolojy and Blood Transfusion popup.description2 Research object: cord blood nuclear cells, cord blood red cell concentrate. Methods: сryobiological, morphological, biochemical, cultural, immunohematological, cytogenetic, molecular genetic. The impact of technological stages of processing cord blood donor samples on their biological usefulness in cryopreservation has been investigated. It has been proved that intra cellular cryoprotectants - DMSO and glycerol have the best protective properties for storage of cord blood red cells at a temperature of -196 ° C. Differences cryoprotective properties of preservative mixtures, which are based crystalloid and colloid solutions have been founded. High efficiency cooling applications using controlled and uncontrolled freezing speed input cord blood nuclear cells in a deep state of suspended animation at a temperature of -196 ° C has been confirmed. Medical and social effect. Medicine. Product Description popup.authors Андреєва С.В. Аношина М.Ю. Балан В.В. Глухенька Г.Т. Дмитренко О.О. Калиниченко Т.О. Мінченко Ж.М. Павлюк Р.П. popup.nrat_date 2020-04-02 Close