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Information × Registration Number 0213U008231, 0111U002204 , R & D reports Title To investigate the efficiency of fetal neuronal stem cells direct differentiation to the GABA-ergic neurons in vitro popup.stage_title Head Tsymbalyuk Vitaliy Ivanovich, Registration Date 26-12-2013 Organization State institution ''Institute of Neurosurgery n.acad. AP. Romodanov NAMS of Ukraine'' popup.description2 The object of investigation was human fetal nerve tissue. The aim of investigation was the induction of nerve stem cells direct differentiation to the GABA-ergic neurons. The methods of nerve cell culture, immunocitochemistry and RT-PCR was used. During experiment nerve cells of human fetuses 9-10 weeks of gestation was tested for capacity to differentiate into GABA-ergic neurons in vitro. Using different neuroinductors and their combination we determined, that combination of extracellular GABA and retinoic acid was most effective of them. We received 19 % of GABA-positive cells in the culture population and 43 % of neuronal fraction. Specific genes expression, connected with GABA-ergic neuron genesis - DLX 2, GAD 65, GAD 67 and GAT 1, was demonstrated too. The obtained data open new perspective for the cell therapy of neurological disorders. So, suspension of nerve cells, enriched by GABA neurons and their determined precursors can be received in culture and using as a material of neurotransplantation. Product Description popup.authors Васильєва І.Г. Галанта О.С. Дмитренко А.Б. Макарова Т.А. Олексенко Н.П. Цюбко.О.І. Чопик Н.Г. Шуба І.М. popup.nrat_date 2020-04-02 Close