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Information × Registration Number 0214U000270, 0114U001100 , R & D reports Title Development and implementation of the methods for diagnostics and gene expression popup.stage_title Head Tkachuk Zenoviy Yuriyovich, Registration Date 04-02-2014 Organization Institute of Molecular Biology and Genetics popup.description2 The innate immune system leads to the formation of protein effector molecules necessary for protection against infectious agents and initiates gene transcription of proinflammatory cytokines , chemokines and other important factors of innate immunity , such as interferon ?, ?, inducible NO - synthase , etc. Gene expression IFN?, IFN, NOS2 were examined by reverse polymerase chain reaction (RT - PCR) in normal lung cells and virus-infected A/FM/1/47 (H1N1) mice C57BL. It was shown that in mice infected with influenza virus , we observed a significant increase in the expression of genes IFN? and IFN?, which both cytokines increased tenfold. Simultaneously healthy mice increased expression of these genes did not occur. We also observed a strong increase in gene expression during infection of mice NOS2 influenza virus compared to healthy animals. Thus, we observed a significant increase in the expression of certain genes for the innate immune system of animals infected with influenza virus . Significant increase (tenfold ) expression of these genes can be used to create a diagnostic system disease caused by the virus . Product Description popup.authors Ткачук Зеновій Юрійович popup.nrat_date 2020-04-02 Close