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Information × Registration Number 0214U000489, 0109U000277 , R & D reports Title Study of cryoresistance mechanisms of placenta tissues and extracts prior to low temperature effect popup.stage_title Head Prokopyuk Olga S., Доктор медичних наук Registration Date 12-03-2014 Organization Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine popup.description2 Research objects were placental cryoextract (PC), fragments of placental tissue, human placental culture, human cord blood serum (HCBS), models of anti-phospholipid syndrome (APS) and general acute cooling (GAC). The research aim was to study the effect of low temperatures on preservation rate of placental materials, examine the effect of cryopreserved biological objects of placental origin (CBOPO) on the proceeding of pathological processes in model experiments. In the work thermometric, cryogenic, biophysical, biochemical, cytological, histological, immune enzyme research methods were used. Results and their novelty. There was designed efficient cryotechnique of preserving CBOPO. There was created and laid for long-term storage the primary collection of CBOPO with determined initial morphofunctional characteristics. It has been proved that low temperature preservation (up to 4 years) of HCBS at -196 C did not cause the changes in protein composition and state of biomacromolecules of HCBS. The study of biological effect of CBOPO in model experiments has shown that preliminary administration of placental cryoextract contributes to the compensation of disorders resulted from GAC effect. Use of HCBS allows the liquidation of characteristic for APS impairments of immune and reproductive systems. Research results can be used to create the stocks of cryopreserved biomaterials to be applied in personalized medicine and as immunobiological preparations in clinical practice. The application field: medicine, veterinary, biology. Product Description popup.authors Воліна В.В. Грошевой М.І. Ліпіна О.В. Мусатова І.Б. Оверко О.М. Погожих О.Г. Присталов А.І. Прокопюк В.Ю. Черемський А.К. Чижевський В.В. Шевченко М.В. popup.nrat_date 2020-04-02 Close