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Information × Registration Number 0214U001036, 0111U002714 , R & D reports Title Develop tools for diagnosis porcine teschovirus encephalomyelitis popup.stage_title Head Bova Tetyana, Registration Date 16-01-2014 Organization The Institute of Agriculture Microbiology and Agriculture Manufacture NAAS popup.description2 Object of research - porcine teschovirus, specific antibodies , test systems. The purpose of research - development of rapid diagnostic teschovirus encephalomyelitis to improve the efficiency of anti-epizootic measures to eliminate these diseases. Research Methods - virology ( isolation and cultivation of virus isolates ) , serological ( antibody display and identification of viral antigens) , physicochemical ( study of the properties of virus isolates ), economic ( cost-effectiveness calculation ) and biometric methods of treatment results. A set of diagnostic tools for the identification of the causative agent of porcine enzootic encephalomyelitis (Teschen disease) on the basis of reverse transcriptase polymerase chain reaction , a set of diagnostics pathogen porcine enzootic encephalomyelitis (Teschen disease) for immunoperoxidase method and improved kit ELISA for the detection of immunoglobulin G virus Teschen disease in the blood serum of pigs and draft documentation on them ( Instructions manufacturing and quality control and card insert ). According to the results of virological monitoring teshovirusnyh encephalomyelitis farms Chernihiv region identified 25 isolates of viruses , of which 80 % related to the disease- Teschen . Established that among pigs and synanthropic animals circulate teshovirusy , antigenically related with the reference strains 1, 3, 6, 11 serotypes . In determining serological pigs profile found that protective antibodies to porcine teshovirus-1have piglets and gilts , vaccinated against Teschen disease vaccine . In 41% of serum levels of intact animals antibodies to the causative agent of Teschen titer of 1:8 in the neutralization reaction , indicating the animal contact with the agent . In terms of cost-effectiveness ( customer value index , an annual economic impact , the total performance index ) developed a test system exceeds the previous development and commercially available counterparts. Product Description popup.authors Дерев'янко Станіслав Васильович Решотько Леонід Миколайович popup.nrat_date 2020-04-02 Close