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Information × Registration Number 0214U001084, 0111U005086 , R & D reports Title Biotechnology - immunological providing selection process of Solanaceous crops identifying new genetic sources to create disease-resistant varieties and hybrids popup.stage_title Head Ivchenko Tatiana Vladimirovna, Registration Date 20-01-2014 Organization Institute of Vegetables and Melon Growing NAAS popup.description2 Object of research: breeding form Solanaceae vegetables. Subject - the improvement biotechnological methods of to create at tissues culture in vitro of new lines for the heterosis breeding. To conduct cell selection to Fusarium for the 13 of tomato genotypes and 15 genotypes eggplant experimental method are installed sublethal concentrations culture FOM F. oxysporum. Selected resistant to FOM calluses clones obtained from plants which - regenerated generations R0 - R1. Receptions were developed to overcome the incompatibility in interspecific hybrids of the genus Solanum in culture in vitro to obtain the source material resistant to biotic and abiotic factors. Established that the when cultivated embryos are at cordate stage on MS medium, modified 0.1 ml / l GK3 and 0.1 ml / l NAA occurs germination 67.7 - 98.7% of embryos eggplant and 10 - 14.3% pepper. To widen the spectrum of available genetic variability and recombination methods are used opportunities of cell selection and experimental mutagenesis. After the treatment of samples of tomato and eggplant effective doses С60 selected cell lines resistant to 30 and 50% FOM F. oxysporum. Received confirmation of saving characteristic functional sterility in tomato of samples generation R0, propagated in culture in vitro Product Description popup.authors Гарт Ольга Юріївна Мірошніченко Тетяна Миколаївна Мозговська Ганна Валеріївна popup.nrat_date 2020-04-02 Close