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Information × Registration Number 0214U001456, 0111U001484 , R & D reports Title To investigate influence of ligands of opioid receptors on adaptive abilities and lifespan of laboratory animals. popup.stage_title Head Dubiley Tetyana Oleksyivna, Registration Date 18-02-2014 Organization State Institution "Institute of Gerontology AMS Ukraine" popup.description2 The influence of mu-opioid receptor blocker naltrexone on the functional parameters, adaptive capacity and life span of Drosophila melanogaster fruit flies and CBA mice were studied. At drosophila males supplementation with naltrexone at concentrations 0,01 - 100 mkg/ml during all imaginal life did not cause significant changes in survival and life span. At females supplementation with naltrexone 48 hours per week at concentration 0,1 mkg/ml starting from day 5 of imaginal life till death led to increase and at concentration 100 mkg/ml - to decrease of survival rate of the flies. The concentration 0,01 and 1 mkg/ml of naltrexone had no effect on this parameter. Supplementation with naltrexone at concentrations 0,1 mkg/ml only during the first half of imaginal life had beneficial impact on the females viability. However naltrexone feeding started from the second half of life had not essential effect on insect survival.At females fruit flies naltrexone at concentration 0,1 mkg/ml enhanced the influence of mu-opioid receptors agonist on locomotor activity, led to decrease of basal metabolism and fertility, slowed the age-related changes of locomotion, increased endurance to the food deprivation and diethyl ether vapour but decrease resistance to the heat shock. At males flies supplementation with naltrexone at concentrations 100 mkg/ml during all imaginal life led to attenuation of age-related changes of locomotor activity at geotaxis test compared with control insects. Body weight was also higher in these flies. The females after treatment with the same dose of drug demonstrated better parameters of locomotor activity in phototaxis test and enhanced resistance to the sleeper effect of diethyl ether compared with control flies. Supplementation with naltrexone at concentration 0,1 mkg/ml starting from day 5 to 7 of imaginal life of drosophila females had no effect on the duration of development of the progeny, offspring sex ratio, but reduced their survival on preimaginal stage. At males and females of the F1 generation revealed increased resistance to the toxic effect of diethyl ether vapor, at females - increased resistance to starvation, but worse, compared with the control, parameters of phototaxis. Survival and life span of male child generation did not change significantly. Females of child generation demonstrated a significant deterioration in the survival rate and a decrease of the maximum life span. Naltrexone treatment (1 mkg/ml in drinking water, once a week during 24 hours) improved the survival and extended maximum residual lifespan of old female mice. The dose 0,01 mkg/ml caused a contrary effect. The results indicate that the use of mu-opioid receptor antagonist naltrexone at low doses at certain stages of development can have significant adaptive and geroprotective effect perhaps due to up-regulation of endogenous opioid activity. Gender peculiarities of the action of naltrexone and its transgenic effects require further study. Product Description popup.authors Мигован С.А. Мороз Є.В. Рушкевич Ю.Є. Тушинська Т.В. Чистова О.О. popup.nrat_date 2020-04-02 Close