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Information × Registration Number 0214U001541, 0111U008741 , R & D reports Title Light-dependent hydrogen production by microalgae (Chlorophyta) and photosynthetic bacteria (Rhodobacter) popup.stage_title Head Zolotareva Elena Kostantinovna, Registration Date 06-03-2014 Organization M.G. Kholodny Institute of Botany NASU popup.description2 Report on research: 36 p. 8 Fig. 3 tab. , 36 references.TObject of study. Microalgae research objects were obtained from natural reservoirs of Ukraine , as well as algological pure cultures of Euglena gracilis and Chlamydomonas reinhardtii Objective: The accumulation , preservation and preparation of microalgal biomass with different water content for further gasification (with the Institute of Gas of NAS ). Determination of the effect of cultural conditions on the biochemical composition of Chlamydomonas reinhardtii cells. Determination of the conditions of formation of the state of the anaerobic culture Euglena gracilis and the potential duration of the H2 photoproduction process. Methods: microbiological, biochemical , physico-chemical, spectral. The samples of blue - green alga Spirulina platensis and green alga Chlorella vulgaris were prepared for further studies of plasma-steam gasification in Gas Institute of NAS of Ukraine. Biomass composition of S.platensis was characterized by higher protein content compared to C. vulgaris. The content of the lipid fraction was higher in the cells C. vulgaris. It was shown that the biochemical composition of C. reinhardtii cells changed significantly during variations of the conditions of cultivation. In the transition to a culture mixotrophic type of nutrition increased the content of protein, amino acids and some cofactors. Microanaerobic state of the cells can be achieved by activation of cellular and chloroplast respiration due to the oxidation of exogenous carbon sources . It is shown that the addition of exogenous ethanol to E. gracilis cells to 12 times stimulates mitochondrial respiration and leads to microanaerobiosis and inducing the production of hydrogen by unicellular microalgae E. gracilis. In contrast , exogenous ethanol negatively influenced the culture of C. reinhardtii. It is rapidly oxidized to acetic acid , which led to a decrease in pH to 3.5, and destruction of culture.the results indicate that the activation of mitochondrial and chloroplast respiration suppresses photochemical reactions in chloroplasts through over reduction of the components of photosynthetic electron-transport chain under anoxic conditions . These conditions contribute to sustainable hydrogen formation in mixotrophic cultures E. gracilis Product Description popup.authors Білявська Нінель Олександрівна Подорванов Володимир Вікторович Поліщук Олександр Васильович Степанов Сергій Степанович Шнюкова Єлізавета Іллівна popup.nrat_date 2020-04-02 Close