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Information × Registration Number 0214U002010, 0113U002332 , R & D reports Title Effect neutron capture agents with photosensitizing properties on cells in vitro under conditions of light and ionizing radiation popup.stage_title Head Lavrenchuk Halina, Registration Date 13-01-2014 Organization State institution "National research centеr for radiation medicine of the National academy of medical sciences of Ukraine " popup.description2 Objective: To investigate the biological effectiveness of the neutron capture agents with photosensitizing properties in experimental model of tumor growth - mammalian cell culture. Object of study: biological effects in experimental model of tumor growth - the culture of mammalian cells under conditions of boron neutron capture agents with photosensitizing properties. Research methods: the cultivation of transplantable cells, cytological, biophysical. An experimental investigation of the modifying effect of boron neutron capture agent with a molecular structure (carborane and porphyrin ) and magnetically controlled nanocomposites containing boron and / or gadolinium on morphological and functional characteristics of the cells in the test-system of culture cell line L929 were carried out, the nature and characteristics of their actions were established. It was found that incubation of the cells with NCA cesium 1 - carbamazepine - klose - dodekarboratum in various concentrations changes in indicators of cells viability were observed. Significant destructive changes (60%) occur when using a concentration of 5 µg/ml. At the lowest concentration (0.05 mg / ml ) changes of viability were not statistically significant. Incubation of cells with NCA solution reduces the viability and increases the amount of polikaryocytes in culture to 40% at all test agent concentration used. Biological effectiveness of nanocomposites based on hydroxyapatite (HA) depended on the structure of the nanoparticles: Gd/HA had no toxic effect, while nanocomposite ferrite gadolinium and HA resulted in weakening the adhesive properties of cells and showed toxicity at higher reactant concentration in the culture medium. Magnetically controlled nanocomposites with gadolinium and boron and those NСA which have been modified by DMSА and DTPA had a greater biocompatibility to cell: incubation of cells with such NCA in the concentration range investigated did not reveal toxicity except for the maximum concentration of ferrite with borron and gadolinium, however, changed cells adhesive properties. For all nanocomposites we observed a decrease in mitotic activity against the same cell population density, which may indicate the timing of cell division. Stabilized by sodium oleate ferrite caused destructive changes in the cell culture at a concentration of only 500 µg/ml and reduced mitotic activity in cell culture in 3-5 times in the entire concentration range. Magnetically controlled nanocomposites containing neutron capture agent (boron or gadolinium) induced apoptosis in cell culture, depending on the reactant concentration. Product Description popup.authors Гапєєнко Дар'я Дмитрівна Литвинчук Христина Михайлівна Оксамитний Віктор Миколайович Олейнікова Тетяна Георгіївна popup.nrat_date 2020-04-02 Close