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Information × Registration Number 0214U002198, 0109U001589 , R & D reports Title Studying of biochemical mechanisms of regulation of Са ions concentration in the smooth muscle cells popup.stage_title Head Kosterin Sergiy Oleksijovych, Registration Date 24-01-2014 Organization A.V.Palladin Institute of Biochemistry of National Academy of Sciences popup.description2 It was proven that the Mg and Са ions are able to change the level of polarization of internal membrane of uterus smooth muscle mitochondria. The increase of Са2+ level in depolarized mitochondria is not provided by functioning of rianodin receptors. With bringing in of two experimental models are fraction of mitochondria and intact myometrium cells - it is proven that the antagonists of calmodulin reduce the level of polarization of myometrium mitochondria membranes. It is proven that active nitrogen and oxygen forms cause Са2+-dependent increase of myometrium sarcolemma polarization, that are the consequence of increase of plasma membrane permeability to К+. The possibility of moderate decline of mitochondria membrane potential registered by means of laser confocal microscopy for the actions of separate derivatives of NO is examined as its protective function in relation to the excessive generation of active forms of oxygen by organoids. Effective inhibitory influence of NPS and NS is shown on Na+,K+-АТР-ase activity in sarcolemma myometrium fraction. Kinetic analysis and results of experiments with the use of ouabain and dithiothreitol allow to pull out supposition, that in basis of such action of the investigated substances can lie chemical modification of thiol groups that are important for the display of catalytic activity to the enzyme. It is proven that calix[4]aren С- 107(10-8-10-4 М) selectively inhibites Na +,K+-АТРase activity: size of imaginary inhibition constant presents 33 ± 4 нМ, the value of Hill coefficient folds 0,38 ± 0,06. Product Description popup.authors Бабіч Лідія Григорівна Бевза Олександр Васильович Вадзюк Ольга Богданівна Векліч Тетяна Олександрівна Горчев Василій Федорович Данилович Ганна Вікторівна Данилович Юрій Володимирович Капля Олександр Андрійович Карахім Сергій Олександрович Лабинцева Раїса Дмитрівна Чуніхін Олександр Юрійович Шкрабак Олександр Анатолійович Шликов Сергій Георгійович popup.nrat_date 2020-04-02 Close