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Information × Registration Number 0214U003330, 0113U002962 , R & D reports Title Adaptive phenomen of hypoxic preconditioning: receptors and signal ways popup.stage_title Head Moibenko Olexii, Registration Date 29-01-2014 Organization Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine popup.description2 Report on research work: 13 p., 4 Fig. Object of research: delayed cardioprotection in rats with hypoxic preconditioning, and its signal ways. Objective: to investigate the effects of delayed functional and structural cardioprotection induced by hypoxic preconditioning, and molecular regulatory mechanisms mediated by kinases. Methods: physiological, biochemical, molecular. The effect of hypoxic preconditioning on signal ways induction, and the development of apoptosis in ischemic-reperfusion injury of rat heart was studied. We found that hypoxic preconditioning was accompanied by protective changes of cardiac function during ischemia-reperfusion, and prevention of reperfusion arrhythmias. After the hypoxic preconditioning, increased to 3.5 - 9 times expression of Akt kinase and to a lesser extent kinase GSK3beta was observed. In the delayed period of preconditioning, in the left ventricle was found significantly more increased Akt expression and in the lesser extent - expression of GSK3beta. In the right ventricle, rapid induction of Akt in the early period was determined. Postishemic development of apoptosis in ventricular myocardium is largely (by 75-94%) prevented in delayed period of hypoxic preconditioning. Severity of anti-apoptotic effects of preconditioning positively correlated with the induction of Akt kinase in the myocardium, and was more significant in the left ventricle of heart. The obtained results may indicate a key role Akt-mediated signaling in the myocardium in anti-apoptotic effects of hypoxic preconditioning. HYPOXIC PRECONDITIONING, CARDIAC FUNCTION, APOPTOSIS, SIGNAL WAYS, Akt AND GSK3beta KINASES, HEART VENTRICLES Product Description popup.authors Лапікова-Бригінська Тетяна Юріївна Портниченко Алла Георгіївна popup.nrat_date 2020-04-02 Close