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Information × Registration Number 0214U003331, 0113U002962 , R & D reports Title Adaptive phenomen of hypoxic preconditioning: receptors and signal ways popup.stage_title Head Moibenko Olexii, Registration Date 29-01-2014 Organization Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine popup.description2 Report on research work: 27 p., 12 Fig. Object of research: delayed cardioprotection in rats with hypoxic preconditioning, and its signal ways. Objective: to investigate the effects of delayed functional and structural cardioprotection induced by hypoxic preconditioning, and molecular regulatory mechanisms mediated by kinases. Methods: physiological, molecular (Western blotting). The effect of hypoxic preconditioning on signal ways activation, cardiac function in rats, and the development of apoptosis in ischemic-reperfusion injury was studied. It was shown that hypoxic preconditioning was accompanied by delayed cardioprotection during ischemia-reperfusion. The systolic pressure in the left ventricle, acceleration of contraction and relaxation of the myocardium were significantly increased, and end-diastolic pressure values were diminished. After hypoxic preconditioning, increase in expression and phosphorylation of Akt kinase by 20% in the right ventricle of the heart, and the kinase GSK3beta by 38.9% in the right and by 14.3% - in the left ventricle was observed, which was accompanied by anti-apoptotic effect. If signaling pathway IGF-PI3K-Akt in isolated heart was inhibited, the performance of delayed cardioprotection of preconditioned hearts were reduced. The results approve the involvement of signaling pathway IGF-PI3K-Akt in delayed cardioprotection induced by hypoxic preconditioning through translational and post-translational activation of Akt and GSK3beta kinases. HYPOXIC PRECONDITIONING, CARDIAC FUNCTION, APOPTOSIS, SIGNAL WAYS, Akt AND GSK3beta KINASES, HEART VENTRICLES Product Description popup.authors Досенко Віктор Євгенович Портниченко Алла Георгіївна popup.nrat_date 2020-04-02 Close