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Information × Registration Number 0214U003345, 0111U000667 , R & D reports Title To develop the technologies of autologous multipotent stromal cell-based products' creating to increase the efficiency of surgical treatment of patients with herniated intervertebral discs popup.stage_title Head Butenko Gennadiy M., Registration Date 29-01-2014 Organization Institute of genetic and regenerative medicine Acad.Med.Sci. of Ukraine popup.description2 The technologies of isolation and culturing of multipotent stromal cells from a human fat (adipose MSCs) and the intervertebral disc nucleus pulposus chondrocytes (NP chondrocytes) have been optimized. The most effective enzyme for cell isolation was 0.2% collagenase IA solution. It was shown at separate and coculture of adipose MSCs and NP chondrocytes that MSCs proliferated the fastest and NP chondrocytes proliferated the slowest. In a group of cocultured cell types an average cell proliferation rate has been observed. Adipose MSCs and NP chondrocytes' cultures actively proliferate in growth medium supplemented with homologous donor AB-serum and AB-platelet lysate. The increased proliferation rate of the considered cell type cultures when cultured under 5% oxygen atmosphere has been observed. It was shown the effective chondrogenic differentiation of adipose MSCs and NP chondrocytes at day 21 with final chondroid formation in standard chondrogenic medium by micromass-culture. Product Description popup.authors Власова О. Галицька С.М. Зубов Д.О. Кирик В.М. Нікольська В.В. Нікольський І.С. Педаченко Є.Г. Тарануха Л.І. Хижняк М. popup.nrat_date 2020-04-02 Close