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Information × Registration Number 0214U003697, 0110U006324 , R & D reports Title Molecular mechanisms of regulation of genes expression involved in riboflavin biosynthesis and transport in flavinogenic yeasts. popup.stage_title Head Boretsky Yuriy Romanovych, Registration Date 17-02-2014 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 Using insertion mutagenesis series of mutants were incapable of active transport of the RF in the cell were selected. It has been shown that one of these mutants damaged gene PGUG_01642 ( orthologs transporter a - glucosides , including maltose in yeast ). Gene deletion PGUG_05894.1 ( orthologs exporter BCRP1 of human) did not lead to changes in the RF ekskretase activity in actively transporting the vitamin strain of P. guilliermondii R93. Introduction of additional copies of the gene into the cells of strain R93 resulted in some mutants BCRP15, to increase the RF - ekskretase activity ( 1.5 -10 times). Introduction of additional copie of the gene PGUG_05894.1 wild-type cells in RF overproducing strain R3 (hit1ura3) in some transformants resulted in a significant increase of flavinogenic activity ( 1.5 - 2 times ), especially when growing them in a medium containing glucose Product Description popup.authors Дмитрук Костянтин Васильович Пиняга Юрій Володимирович Сибірний Андрій Андрійович Фаюра Любов Романівна Федорович Дарія Василівна Яцишин Валентина Юріївна popup.nrat_date 2020-04-02 Close