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Information × Registration Number 0214U005252, 0112U004187 , R & D reports Title Structural changes in the lungы under conditions of endogenous intoxication, which is caused by a burn of the skin, and its correction by domestic infusion preparations Lactoprotein With Sorbitol and HAES-LX-5% (experimental study) popup.stage_title Head Prokopenko Sergey Vasilievich, Registration Date 04-12-2014 Organization Vinnitsa National Pirogov Memorial Medical University popup.description2 Applied innovative method for assessing DNA fragmentation in cells of the lungs, as an essential component of apoptotic cells in burn disease - by flow cytometry DNA. This method of determining DNA fragmentation has significant advantages over the colorimetric method and fluorescence microscopy (eg, "comet assay" using elektoforezu in agarose mikroheli, TUNEL using the enzyme terminal transferase or DNA polymerase - ISNT et al.), Namely the order increasing number of analyzed objects one study, the possibility of quantitative rather than qualitative indicators of DNA fragmentation, greater speed preparation of cell suspensions or the nuclear and consequently much less negative effect on the cells during specimen preparation. So far, the definition of DNA fragmentation by flow cytometry mainly used to identify the proportion of apoptotic cells in cell cultures in vitro. We first results obtained in experimental conditions in vivo, both in normal and in burn disease and its correction, which is the newest. The use of innovative methods of flow cytometry for DNA cell cycle assessment in lung cells in burn disease. This method has significant advantages over commonly used in practice, morphological, immunohistochemical and radiometric methods - is the most informative, allowing for a greater amount of the investigated material quantify cell division phases of the cell cycle. We first results obtained using this method for the evaluation of the cell cycle in the lungs in experimental conditions, burn injuries of the skin. Product Description popup.authors Є. Міронов І. Булько І. Гунас І. Дзевульська І. Кирилюк І. Кириченко І. Паламарчук І. Стефаненко І. Черешнюк І. Щерба А. Гаврилюк А. Король А. Очеретнюк А.Семенеко Б. Кондрацький В. Корінний В. Титаренко Г. Блажієвська Г. Галунко Г. Даценко Д. Лисенко Е. Черкасов К. Паламарчук Л. Клімас Л. Присяжна Л. Сарафинюк М. Зорич М. Костенко Н. Камінська Н. Очеретна О. Герасимова О. Кирилюк О. Ковальчук О. Макарова О. Маліков О. Паламарчук О. Семененко О. Ситнік О. Ходаковський О. Шніпор О. Яковлева Р. Матківська Р. Радьога Т. Вітрук Т. Лахтадир Т. Поліщук Ю. Фурман popup.nrat_date 2020-04-02 Close