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Registration Number

0214U005434, 0110U005689 , R & D reports

Title

Biological activity and safety of nanomaterials that could be used in development of vector systems of antitumor drugs

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Head

Chekhun Vasyl Fedorovych,

Registration Date

29-12-2014

Organization

R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology

Description

We performed series of experimental studies, aimed at determination of features and mechanisms of action of metal-containing materials of different origin on tumor cells in vitro and in vivo, particularly ferromagnetic nanoparticles, magnetic fluid and colloid gold. We proposed a systemic multi-level approach for determination of nanomaterials safety degree, which consisted of wide spectrum of criteria and methods of study, and was performed according to the following algorythm: determination of degree of nanomaterials cytotoxic action on tumor cells by cytomorphological indices; analysis of genotoxic effects of nanomaterials on tumor cells (micronucleus test, quantitative evaluation of DNA Comet Assay); determination of apoptosis level in tumor cells; evaluation of reactive oxygen species and peroxides generation as products of free-radical metabolism; study of mitochondrial potential; determination of SH-groups number as an index of detoxication system activation; evaluation of lipids ratio in tumor cells. Results of the project were presented in methodical recommendations: "Criteria and methods of evaluation of biological safety of metal-containing nanomaterials in development of vector antitumor systems" (2014). Results were published in 5 articles in Ukrainian and international journals with high impact factor and in 4 Ukrainian patents (2011-2014)

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Бурлака Анатолій Павлович
Ганусевич Ірина Іванівна
Демаш Дмитро Валерійович
Лозовська Юлія Валеріївна
Лук'янова Наталія Юріївна
Мельник Наталія Миколаївна
Налєскіна Леся Анатоліївна
Стеблянко Тамара Ісаківна
Тодор Ігор Миколайович
Трембач Людмила Валентинівна
Чехун Василь Федорович
Швець Юлія Вікторівна