Search academic texts

Information × Registration Number 0214U005872, 0113U006456 , R & D reports Title Investigation of the direct and indirect action of the radiation exposure on the biological systems popup.stage_title Head El'ska Ganna Valentynivna, Доктор біологічних наук Registration Date 24-12-2014 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 Aim of the project - to study the molecular mechanisms of the tautomeric transformation of the wobble Gua·Thy and Ade·Cyt DNA base pairs into the Gua*·Thy and Ade·Cyt* mispairs involving mutagenic tautomers of the bases, respectively, induced by the recognition center of the Watson-Crick base pairs of the replicative DNA polymerases. Methods of investigation - novel methods of the computer simulation of a high level of complexity, in particular quantum-mechanical (QM), and Bader's topological analysis of the electron density (QTAIM). By combining QM and QTAIM methods of investigation and by using the methodology of the scans of the basic physico-chemical characteristics of the double proton transfer along the internal reaction coordinate, for the first time we have elucidated physico-chemical mechanism of the mutagenic tautomerisation of the Gua·Thy and Ade·Cyt base pairs. For the first time a new physico-chemical mechanism of the origin of the spontaneous transitions has been proposed and substantiated. It consists in the tautomerisation of the pyrimidine bases of the wobble Gua·Thy and Ade·Cyt base pairs with their transformation into the Gua*·Thy and Ade·Cyt* mispairs, respectively, induced by the interaction with the recognition center of the correct pairs of the nucleotide bases of the replicative DNA-polymerases. Product Description popup.authors Єльська Ганна Валентинівна Броварець Ольга Олександрівна Говорун Дмитро Миколайович Горб Леонід Григорович Кухта Павло Андрійович Пальчиківська Лариса Гнатівна Потягайло Андрій Любомирович Самійленко Світлана Панасівна popup.nrat_date 2020-04-02 Close