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Information × Registration Number 0214U005901, 0110U004744 , R & D reports Title Molecular and genetics markers identification for epithelian cancer diagnostics. popup.stage_title Head Kashuba Vladimir Ivanovich, Registration Date 26-12-2014 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 Analysis by NotI-microarrays identified 22 genes which methylation frequency and / or deletions are higher by more than 17% in tissues from CCRCC compared to healthy tissue of the kidney. Most of these genes was shown for the first time in kidney carcinogenesis. The NotI-microarrays data were confirmed by bisulfitne sequencing. It was shown that methylation of chromosome 3 genes (especially short arm) is a frequent event in CCRCC. Expression analysis with Q-PCR for 6 genes with high frequency M / D also showed high (20-92%) reduction in expression. For one of them, ALDH1L1, there was a positive correlation between the decrease in mRNA levels and disease stage for CCRCC and papillary carcinoma. The results allow us to assume that the established panel on the basis of these 6 genes will allow to detect CCRCC in a kidney biopsy material with an accuracy of 87%. Product Description popup.authors Геращенко Ганна Володимирівна Гордіюк Василь Васильович Кондратов Олександр Георгійович Панасенко Григорій Володимирович Руденко Євгенія Євгенієвна popup.nrat_date 2020-04-02 Close