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Information × Registration Number 0214U005903, 0110U006122 , R & D reports Title Designing and efficiency research of the recombinant baculovirus vectors for gene and vaccinotherapies popup.stage_title Head Strokovskaya Lyudmila Ivanovna, Registration Date 26-12-2014 Organization Institute of Molecular Biology and Genetics popup.description2 This year the study of gene therapy with recombinant baculovirus (RB) and cell vectors with cytokines genes on the proliferation of melanoma tumor cells and MM4 Lewis adenocarcinoma in vivo was continued. Also the expression of some genes which changed expression in response to interferon in many cell types was examined. According to preliminary data of ?-Sma and Gre1 gene expression, mouse fetal fibroblasts have signs of MSC-like cells and their use as a model to study the interaction between stromal cells / tumor cells may be promising. The possibilities of usage of the mouse fetal fibroblast transduced with baculovirus vectors contained human interleukin 21 and mouse ?-interferon genes and also recombinant baculovirus with above named target genes for tumor growth inhibition in vivo were shown. It was found, that "vector" cells (fetal fibroblasts) transduced with recombinant baculovirus are more effective in comparison with recombinant baculovirus themselves in anticancer therapy of Lewis adenocarcinoma and mouse melanoma. Interferon-?, which was synthesized by transduced fibroblasts completely inhibited cell proliferation in vivo and adenocarcinoma tumors didn't occur in experimental animals.5481 Product Description popup.authors Анопрієнко О.В. Вагіна І.М. Гончарова Л.О. Зінченко В.А. Захарук О.А. Кіхно І.М. Чащина Л.І. popup.nrat_date 2020-04-02 Close