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Information × Registration Number 0214U006762, 0110U004660 , R & D reports Title Improvement the efficiency of plant cells Agrobacterium-mediated transformation by protein kinase inhibitors popup.stage_title Head Yemets A. I., Registration Date 14-02-2014 Organization Institute of Food Biotechnology and Cenomics of the National Academy of Sciences of Ukraine popup.description2 An influence the different concentrations of protein kinase (5-100 µM W7 and 10-200 µM trifluoperazine) and protein phosphatase (0,1-3 µM sodium orthovanadate) inhibitors on transient and stable Agrobacterium-mediated transformation of Nicotiana tabacum plant explants was investigated. It has been established that an application of trifluoperazine only is the most effective for genetic transformation. Thus, 10 and 25 µM trifluoperazine increased stable transformation frequency up to 25% as compared to control. The results of transient transformation shown, that 10 µM is the most effective trifluoperazine concentration leading to the highest intensity of reporter gus gene expression. Product Description popup.authors Блюм Я.Б. Карпов П.А. Танасієнко І.В. Федорчук В.В. popup.nrat_date 2020-04-02 Close