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Information × Registration Number 0214U006952, 0113U006054 , R & D reports Title Development of repairing technology of liver injury by MSC transplantation popup.stage_title Head Rymar Svitlana Yuhymivna, Registration Date 15-04-2014 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 Any hazard factor that consistently affects the liver, induces fibrosis, which transforms into cirrhosis if the action continues. Animal models of liver fibrosis are the main tool for its study and for development of approaches for the treatment of advanced fibrosis and cirrhosis by transplantation of mesenchymal stem ( stromal ) cells. Induction of liver injury of laboratory Wistar rats was performed by intraperetonial introduction of 30% oil solution of CCl4 per 100 g weight once a week. Every three weeks the condition of the liver was analyzed by biochemical blood tests for ALT and AST, histological analysis of liver parenchyma performance, investigation of the expression levels of EGF (epidermal growth factor ), Igfbp2 ( 2nd linking insulin like factor protein), eNOS and iNOS ( epidermal and inducible nitric oxide synthase genes). The dynamics of changes of all these parameters was studied for 18 weeks and showed that after 3 weeks of induction , there were signs of inflammation of the liver , and after 18 weeks transition of fibrosis into micronodular cirrhosis occurred. Thus, the model for liver damage was obtained and the dynamics of its development was studied. All the methods used in the study adequately defined different stages of liver disease and can be used to evaluate the liver state during the development of the lesions correction method by transplantation of MSCs . Product Description popup.authors Дерябіна О.Г. Драгулян М.В. Ковальчук М.В. Похоленко Я.О. Шпильова С.П. popup.nrat_date 2020-04-02 Close