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Information × Registration Number 0214U008365, 0110U006191 , R & D reports Title Application of effective oligoelectrolyte systems for genes delivery into cells for genetic transformation of plants popup.stage_title Head Stoika Rostyslav, Registration Date 22-12-2014 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 It was determined the possibility of poly-DMAEM carriers using for genetic transformation of protoplasts of tobacco Nicotiana tabacum L. It was found that carrier 83/6 at a concentration of 0.1% is the most efficient for genetic transformation of protoplasts of tobacco N. tabacum. This polymer indicated low cytotoxicity for protoplast combined with effective genetic transformation. Less favorable to transform protoplasts of tobacco are polymers of BG-2f, MP-1ta BG-2q types at the concentrations of 0.5 and 0.25%, because, in addition to an intense expression of the reporter gene YFP in single cells it was observed minor morphological changes - aggregation, loss of spherical plasma membrane integrity violations and structured internal contents of cells. Product Description popup.authors Бойко Наталія Миколаївна Фінюк Наталія Степанівна popup.nrat_date 2020-04-02 Close