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Information × Registration Number 0214U008576, 0113U003652 , R & D reports Title Development of enzyme-linked immuno sorbent diagnostic kit to determine 25OHD3 as a marker of bone diseases. popup.stage_title Head Veliky Mykola Mykolajovytch, Registration Date 27-06-2014 Organization A.V.Palladin Institute of Biochemistry of National Academy of Sciences popup.description2 The development of methods and 25-hydroxycholecalciferol (25OHD3) coupling to hemocyanin (KLH) as an immunogen support were performed. 25-Hydroxyvitamin D3-3-hemisuccinate-KLH was synthesized. For this synthesis 25-hydroxyvitamin D3 was chemically activated at position 3 via the intermediate steps of 25-hydroxyvitamin D3-3-hemisuccinate and 25-hydroxyvitamin D3-3- hemisuccinate-N-hydroxysuccinimide ester preparation. The resulting conjugate was purified by column chromatography Econo-Pac 10DG. The reliability and efficiency of the synthesized products were confirmed by thin layer chromatography after conjugate saponification. The immunization of rabbits with 25-Hydroxyvitamin D3-3-hemisuccinate-KLH is currently carrying out. Product Description popup.authors І.О. Шиманський А.В. Хоменко Л.І. Апуховська Л.Б. Дробот О.О. Лісаковська С.В. Комісаренко popup.nrat_date 2020-04-02 Close