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Information × Registration Number 0215U000282, 0110U000404 , R & D reports Title Study of the mechanisms of cryodamage and cryoprotection of immobilized cells to increase their preservation during cryopreservation and lyophilization popup.stage_title Head Vysekantsev Igor Pavlovich, Registration Date 05-02-2015 Organization Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine popup.description2 Research objects were yeasts S. cerevisiae, S. boulardii; bacteria E. coli M-17, B. bifidum 1, L. delbrueckii subsp. bulgaricus; cell cultures SPEV, L 929. Research aim was to study cryodestruction and cryoprotection of immobilized cells on / in the carriers and preservation of their biological properties after low temperature storage. Methods: microbiological, cultural, biochemical, biophysical, light, fluorescent and electron microscopy, modeling of pathological states of experimental animals. Equipment: fluorescent and scanning electron microscopes, spectrophotometers, flow cytometer, a programable freezer of bioobjects "Cryoson BV-6" (Germany), equipment for lyophilization. Experimentally proved cryopreservation modes of research object - immobilized microorganisms on / in carriers. Established that immobilization on / in carriers and low temperature storage do not cause significant changes in the biological properties of microorganisms. Immobilized probiotics onto sorbents exhibit more pronounced therapeutic effect during the correction of experimental dysbiosis. It is shown that immobilization in polysaccharide gels protects internal structure and the MTC and the cell wall of yeast cells against cryodamages. It was found that after lyophilization in the media with the addition of sodium alginate gel beads and bifidobacteria and lactobacilli retain high viability and biological properties. The cryopreservation technology of cell cultures in medium without serum with the addition of DMSO gel and MC. The results will be used in future studies to create immobilized immunological agents, methods of long-term storage. Product Description popup.authors Євтухова Людмила Іванівна Іванко Людмила Сергіївна Абрафікова Лілія Генадіївна Ананьїна Ганна Євгенівна Артуянц Анастасія Юріївна Бабінець Ольга Михайлівна Буряк Ірина Алімівна Висеканцев Ігор Павлович Гуріна Тетяна Михайлівна Дорофеєва Тетяна В'ячеславна Калашнікова Марина Миколаївна Кудокоцева Ольга Валентинівна Марценюк Валентина Пилипівна Онасенко Олена Станіславівна Пішко Ольга Володимирівна Петренко Тетяна Пилипівна Пономарьова Вікторія Леонідівна Сергієнко Алла Миколаївна Шатілова Людмила Євгенівна Щеглов Андрій Валерійович popup.nrat_date 2020-04-02 Close