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Information × Registration Number 0215U001161, 0114U006074 , R & D reports Title Controlling the processes of prolifetion and differentiation of progenitor cells on surface of nanostructured films by means of their modificationof ligating mixtures popup.stage_title Head Goltsev Anatoliy Nikolayevich, Registration Date 30-01-2015 Organization Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine popup.description2 Research object was the bone marrow cells of СВА/Н mice of 15-week age. This research was aimed to study the interaction of cells with nanostructured surfaces of doped dielectric and electret films and to select the coatings on which their adhesion and proliferation were strengthened or weakened, as well as to design the model of cell adhesion on surface with the certain nanostructure and electret state. Research methods were as follows: flow cytometer analysis, confocal and inverted microscopy, in vitro culturing. The dependency of adhesive potential of heterogeneous population of bone marrow (BM) cells on the properties of oxide nanocoatings was demonstrated. When culturing cells on glass and Al2O3 no differences in their capability to adhesion were observed. In contrast the Ta2O5 nanocoating reduced twice the adhesive properties of the studied cells. The Al2O3 coating stimulated a proliferative activity of BM adhesive cells, that was testified by an increased increment rate of cell population as compared to the glass. Treating this coating with electrons in contrast to that with ions resulted in even greater stimulation of functional properties of the studied cells. The doped dielectric and electret Ta2O5 coating prevented not only the adhesion, but the proliferation of adherent cells as well. The findings of flow cytometry analysis clearly testify to the capability of Al2O3 coating to selectively enrich the BM cultures even at the first passage with the cells having phenotypic sings of mesenchymal stromal cells. There was designed the model of cell adhesion on extracellular two-dimensional matrix, which was entirely consistent with the current notions about mechanism of cell moving on this matrix. Basing on the research performed there were selected the nanocoatings, either stimulating or weakening the adhesion and proliferation of BM cells, that was the reason to use nanocomposite oxide coatings in medicine. The electret Al2O3 coating is capable to increase adhesive and proliferative potentials of mesenchymal stromal cells, that is necessary for obtaining a "therapeutic dose" to be introduced into the site of traumatic injuries and congenital orthopedic defects. The Ta2O5 nanocoating, possessing the capability to prevent adhesion of cells of any origin may be used in vascular surgery. Product Description popup.authors Гордієнко Євгеній Олександрович Дубрава Тетяна Георгіївна Останков Максим Вадимович Рассоха Ірина Вікторівна popup.nrat_date 2020-04-02 Close