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Information × Registration Number 0215U001418, 0111U008741 , R & D reports Title Light-dependent hydrogen production by microalgae (Chlorophyta) and photosynthetic bacteria (Rhodobacter) popup.stage_title Head Zolotareva Elena Kostantinovna, Registration Date 27-02-2015 Organization M.G. Kholodny Institute of Botany NASU popup.description2 Report on research: 33 p. 6 Fig. 3 tab. 28 references.The object of study. Microalgae research objects were obtained from collection of microalgae in M.G.Kholodny Botany Institute of Natl. Acad. Sci. of Ukraine , as well as isolated culture of purple photosynthetic bacteria Rubrivivax gelatinosus. The aim of the study: The study of [NiFe]-hydrogenases of purple bacteria Rubrivivax gelatinosus, tolerant to O2. Determination of the effect of trace elements on the formation of the anaerobic state of the Euglena gracilis culture and potential duration of the process of H2 photoevolution. Methods: microbiological, biochemical, physical, chemical, spectral. The effect of O2 on the growth Rubrivivax gelatinosus in the presence of CO was studied in the light and in the dark at variation of oxygen content in the medium. The results allowed us to estimate the stability of the enzyme systems of purple bacteria, including hydrogenase to the inhibitory action of oxygen. Hydrogenase activity of Rubrivivax gelatinosus was determined in the reaction CO + H2O =CO2 + H2 at variation of the oxygen level and tolerant to O2 [NiFe] - hydrogenase of R. gelatinosus was characterized. Structural changes in the R. gelatinosus cells were studied by electron microscopy. The basic conditions of application of photosynthetic purple bacteria (Rhodobacter) for H2 production were proposed according to experimental studies. Sodium sulfite was tested as a reagent improving the efficiency of Chlamydomonas reinhardtii cells in photobiological hydrogen generation. The contribution of chloroplast respiration and dark reduction of plastoquinone in the formation of Euglena gracilis anaerobic state and the effect of trace amounts of vanadium on the growth and induction of hydrogen generation was determined for the E. gracilis cells. Product Description popup.authors Подорванов Володимир Вікторович Поліщук Олександр Васильович Степанов Сергій Степанович popup.nrat_date 2020-04-02 Close