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Information × Registration Number 0215U001495, 0112U001026 , R & D reports Title Elaboration ofthe vertebrateclonal progeny obtaining technology using hemiclonal interspecific hybrid crossing (by the example ofgreen frogs) popup.stage_title Head Shabanov Dmitro, Registration Date 04-03-2015 Organization Kharkov National University named after V.N. Karazin popup.description2 Targets of the research — natural hemiclonal interspecies hybrids (in the case of Pelophylax esculentus complex); Pelophylax esculentus clones, that would be obtained during the research. Study objectives — obtaining and studying the vertebrates’ clonal litters by hemiclonals’ crossing; collecting the females and males clones that will reproduce in crossings. Methods: — Collection of breeders of Pelophylax esculentus complex representatives in natural habitats, their artificial crossing using hormonal stimulation under the condition of zooculture, clonal litters obtaining and growing; — Analysis of the Pelophylax esculentus complex population systems natural diversity, determining of their biocenotic and geographical causality; — Qualitative definition of population system type using composition of forms included in it; — Estimation of population systems' magnitude and composition using marking and re-capture method; — The primary determination of individuals' species based on the external morphology; — Rapid determination of individuals' ploidy using cytometry; — Comparative ecological, morphological, biochemical and physiological studies of representatives Pelophylax esculentus complex; — Individuals' genotyping using flow cytometry and protein electrophoresis; — Karyoanalysis of macerated tissues squash preparations, pipette metaphase plates and cells; — Determination of genome gametes structure using flow cytometry, protein electrophoresis, lampbrush chromosome analysis and analysis of crossings; — Simulation modelling and experiments with simulation models; statistical analysis of simulation results (using Principal Component Analysis); — Comparison of simulation results with empirical data, formalization of empirical and literature data, etc. The variety of HPS (hemiclonal population systems of frogs) from Left Bank forest-steppes of Ukraine and neighboring regions were described in the course of project; 5 sub-regions, that differ in composition of HPS, were distinguished; the features of gametogenesis and reproduction of interspecific hybrids, specific to the region of studies, were determined; the principles of green frogs HPS simulation modelling were developed and simulation models of their transformations were created; the dynamic typology of green frogs HPS consisting of diploid representatives were developed; the typology of biosystems stability conditions was proposed; the conditions of sustainable existence of natural and artificial HPS consisting of clones were determined; the hypothesis that explains the origin of hemiclonal inheritance as adaptation to homoeologous recombination was created; the artificial crossing technology and quality evaluation of males-breeders in laboratory zooculture conditions were developed; the clonal litters of green frogs were obtained. Product Description popup.authors Є. Скоробогатов В. Строїлов Д. Шабанов К. Сєдова М. Кравченко Н. Токарська О. Бірюк (Михайлова) О. Коршунов О. Мелешко О. Усова popup.nrat_date 2020-04-02 Close