Search academic texts

Information × Registration Number 0215U003152, 0113U000460 , R & D reports Title Methodological approaches elaboration to the matter of biological preservation of tissue typing of cord blood samples providing as a basis for the creation of donor hematopoietic tissue cryobanks popup.stage_title Head Kalynychenko T.O., Доктор медичних наук Registration Date 22-01-2015 Organization Kiev Research Institute of Haematolojy and Blood Transfusion popup.description2 Research object: cord blood nuclear cells, cord blood red cell concentrate. Purpose - to determine the optimal freeze - dekonservatsiyi hematopoietic tissue donor cord blood for preservation of biological potential.Methods: сryobiological, morphological, biochemical, cultural, immunohematological, cytogenetic, molecular genetic. The effectiveness of freeze human cord blood erythrocytes (HCBE) at ultralow temperature with extracellular and intracellular cryoprotectants as well as their mixtures has been examined. It has been proved that the combination of the polymer - PVP and penetrating cryoprotectant - DMSO at the low concentration is an effective prerequisite for the protection of HCBE at ultralow temperature in terms of receiving of the high quantitative and qualitative material characteristics. The impact of technological depreserve stage of umbilical cord blood hematopoietic tissue samples at its biological usefulness has been studied. The feasibility of using low concentrations of DMSO 5% by freezing in liquid nitrogen vapor has been shown to hemopoietic tissue cord blood bank functioning because of the possibility of exclusion of the preparatory phase for enter the body of the recipient. Medical and social effect. Medicine. Product Description popup.authors Андреєва С.В. Аношина М.Ю. Балан В.В. Глухенька Г.Т. Гордієнко А.І. Дмитренко О.О. Калиниченко Т.О. Мінченко Ж.М. Павлюк Р.П. popup.nrat_date 2020-04-02 Close