Total number
Full text
Total number
Full text
Number of local repositories
Full text
0215U003221,
0113U002317
,
Definition of the role of genetic and epigenetic alterations in the genome of somatic cells in the pathogenesis of chronic lymphoproliferative diseases, their diagnostics and clinical significance
Bebeshko Volodymyr; Minchtnko Zhanna,
21-01-2015
State institution "National research centеr for radiation medicine of the National academy of medical sciences of Ukraine "
Object of study - biological material of 107 patients with multiple myeloma, chronic lymphocytic leukemia, non-Hodgkin's malignant lymphoma. Aim - to study the epigenetic status of cell cycle regulation genes and of cytokine production genes and to analyze rearrangement fragments of immunoglobulin heavy chains genes in patients with chronic lymphoproliferative disorders. Methods - molecular cytogenetic, cytogenetic, immunogenetic, immunological, hematological, cultural and statistics. Results. The abnormalities of chromosome 17 in the locus of TR 53 gene were found in 36 of 107 (34%) CLPD patients as a result of molecular cytogenetic analysis of bone marrow, lymph nodes and peripheral blood. Clonal deletion of the TP 53 gene was revealed more often. Hiperaneuployidiyi of chromosome 17 were registered in 14% of patients with CLPD. 49 (46%) patients had not changes of genetic structures. Translocations t (4;14), t(11;14) of genes FGFR3 and CCND1 were not found in B-CLL patients. IGH aneuploidy were identified in 4 patients of 10. Translocation t(11;14) was registered in 4.3% of MM patients. A retrospective study of 17 samples of paraffinized biopsy material from lymph nodes of DBCL patients and three samples - of Burkitt's lymphoma patients showed that MYC rearrangements were registered for Burkitt's lymphoma in two of the three patients. Translocation t(8;14) was found in two of seventeen patients with DKVL. Aneuploidy of chromosomes 8 and 14 was registered in six of twenty patients. The need to identify differential prognostic markers of the disease, including chromosomal rearrangements, was confirmed. It was shown that the presence in a HLA-genotype alleles HLA-A*24; HLA-B*52; HLA-C*06; DRB1*08, HLA-DQB1*05 and HLA-DQB1*06 is a factors of genetic susceptibility to the MM. Allele HLA-A*01 has a protective function. The selection of the primers with optimum number of CG dinucleotides located mainly in promoter areas of these genes was made. Methylation pattern of genes CCND1, TNF-alpha and IGHJ1 in genomic DNA of patients satisfied the reference DNA, changes were not detected. It was proved that before chemotherapy in patients with MM concentration of IL-6 and TNF-? in a peripheral blood serum significantly exceeded one in healthy individuals. The highest concentrations of TNF-? and IL-6 were identified in MM patients with resistance to chemotherapy. The number of CFU-F in multiple myeloma petients depended on the form and stage of disease.
Балан Валентина Володимирівна
Дмитренко Олена Олександрівна
Мішаріна Жанна Анатоліївна
Сітько Валентина Віталіївна
Шляхтиченко Тетяна Юріївна
2020-04-02
Updated: 2025-12-26
Saving a scientific data set occurs in two stages:
1.) Creating a data set
Enter the name of the set, a brief description of the set (annotation), and click the “Create set” button. After that, a draft data set will be automatically created, which you can save for further work with the set. The system will redirect you to the “Data set information” page.
2.) Filling in the data
On the “Data set information” page, you need to fill in all the fields related to the data set and upload the files.
Please note! Each file is uploaded separately. This is done to ensure the stable operation of the system.
Once you have filled in the information about the scientific data set and added the necessary files, you can save the draft or send the data to the system.
Please note! Once the data has been sent, you cannot change it.
Drafts are necessary for storing the data set and editing it before sending.
All accounts on the NRA website go through a standard registration procedure and are verified. However, in order to obtain additional features, such as publishing open reviews of academic texts, verification by affiliation is required.
To obtain the status “verified”, you must change your primary email address to a corporate one, which is tied to a scientific or educational institution.
For example: user@ukrintei.ua, user@nas.gov.ua
After confirming your corporate email address, your account will be automatically verified by affiliation.
If your email address is corporate, but your account does not have a verification mark, you need to write to us at the email address NRAT@ukrintei.ua. After verification, we will add your address to the database, and your account will be verified.
After completing the verification procedure for affiliation, you can change the primary email address to a convenient one for you, without losing your status during the current verification.
You only need to verify for affiliation once.
* We use corporate addresses from the Unified State Database on Education and the State Register of Scientific Institutions Supported by the State
To search for news published on the official website of NRАT in the “News” section, you need to enter one word, several words, or an exact phrase in the search field. Different terms are separated by spaces.
The search is performed everywhere – in the title and the news text.
For a more comprehensive result, it is recommended to use word stems (parts of words without endings).
When using multiple words for the search, they are combined using one of the logical methods described below.
By default, the logical connector “and” is used. In this case, search results will include all news releases that contain the specified words, even if they are located separately from each other and are in different parts of the text.
Example: Search phrase: OECD report. Search result: all news containing the words “report” and “OECD” in the text and in the title, both together and separately.
If a phrase or phrase is enclosed in quotation marks (“), the results will include all publications where this exact phrase or phrase is found.
Example: Search phrase: “OECD report.” Search result: all news containing the exact phrase “OECD report.”
If you know the publication date of the news or are interested in a specific time frame in which they were published on the website, you can select such a date or date range in the additional field that looks like a calendar. The date is entered in the format year-month-day and confirmed by clicking on the corresponding date in the calendar dropdown. You can enter a date or choose one by browsing through the calendar by months and years. By default, the interval starts from the oldest date (2018-01-23) and ends with the current date.
You can also use one or more hashtags, which are located under the search field on the right side of the “News” page.
Please note: The search for academic texts is carried out in a separate search window at the following link https://nrat.ukrintei.ua/searchdb.
