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Information × Registration Number 0215U003403, 0113U003835 , R & D reports Title Increasing efficiency of recombinant scFv-antibodies expression and facilitating their detection in immunoassay. popup.stage_title Head Oliinyk Olena Serhiivna, Registration Date 29-01-2015 Organization A.V.Palladin Institute of Biochemistry of National Academy of Sciences popup.description2 Single chain variable fragment (scFv) is useful and effective recombinant antibody format. But detection of scFv and their complexes with antigens is problematical: prevailing systems for detection have some drawbacks and are not universal We constructed fusion proteins, which contain sequences of scFv and staphylococcal protein A fragment or fluorescent protein mCherry. It was shown, that scFv fused with protein A and their complexes with antigens were effective recognized by labelled immunoglobulins and that yield of active functional fusion proteins in a similar expression system was significantly higher than the yield of free scFv. ScFv to growth factor HB-EGF fused to mCherry were functionally active in flow cytometry and immunofluorescent microscopy. This new fusion antibody Anti HB-EGF ScFv-mCherry can test HB-EGF positive cells. Product Description popup.authors Короткевич Н.В. Лабинцев А.Ю. popup.nrat_date 2020-04-02 Close