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Information × Registration Number 0215U004177, 0112U005549 , R & D reports Title "Investigation of influence metal and carbon nanomaterials as matrix for differentiation growing cells in vitro" popup.stage_title Head Sydorenko Myhail V., Registration Date 12-02-2015 Organization "Department of Biotechnical Problems in Diagnostic of Institute of Problems of Cryobiology and Cryomedicine National Academy of Sciences of Ukraine" popup.description2 121 p., 34 fig., 8 photos, Table 7., 145 literature sources. The object of study - onion like carbon, ultra nanodiamonds, single nanotubes and fullerenes, tumor cell lines of breast adenocarcinoma (MCF-7), a line of laboratory mice Balb / c. The goal - a comparison of the qualities of carbon nanomaterials (diamond and onion like carbon nanotubes, fullerenes) as an adhesive matrix for cell cultivation with transformable phenotype and study the features of the morphogenesis of cells in a modification of the cell substrate nanostructures. Determine ways of application of carbon nanomaterials with different physical and chemical characteristics. Research methods - methods of cell culture, cyto-morphological, ELISA, immunocytochemical staining, biochemical analysis, methods of spectral studies - Fourier spectrometry, colorimetric analysis. The study examined the impact of four forms of carbon nanomaterials (UDD - ultra disperse nanodiamonds, fullerenes, VHV - single-walled carbon nanotubes and APs - onion like carbon) on the clonogenic activity of tumor cell line MCF-7. Namely, it was found that nanosized carbon structures aggregates APs UDD, C60 and DMS affects the proliferation, adhesion of cells in culture and formation of multicellular aggregates can thereby determine the further development of the cell population. Thus, APs and UDD aggregates may reduce the adhesion of cells to the substrate and contribute to increase the number of small spheroids cell (up to 1 ? 10-3 mm3). C60 and DMS, on the contrary, create the conditions for the formation of cell aggregates of up to 5 ? 10-3 mm3. These findings are also supported by statistical calculations of the correlation coefficient and concentration of nanomaterials size tumor spheroids with co-cultivation, which for APs is "-0.84" UDA "-0.81" fullerenes "0.5", and for ONT "0 86 '. At the same time, the UDD not affect the expression of tumor epidermal growth factor receptor, E-cadherin, cytokeratins (clone AE1 / AE3) and the estrogen receptor. Level genotoxic damage of tumor cells can not be repair by incubating with the UDD at a concentration of 80-120 mg / ml was only 35% of the targets. Intraperitoneal administration to laboratory mice UDD at a concentration of 0.1 mg / 100 .mu.l / animal did not lead to intoxication or significant deterioration in animals. Observed activation of liver enzymes ALT, AST, and a slight increase in LDH. At the same time UDD have a very valuable physico-chemical parameters as a positive Zeta potencial (40mV), chemical inertness, absorptive capacity, which creates conditions for their use as an effective and low-toxic pharmaceutical carriers. Fullerenes like UDD affect clonogenic potential of tumor cells. Because the genetic influence on the activity of the unit cells and proliferation has several stages. In the first stage after incubating C60 introducing into culture medium an accelerating cell growth and increase the number of cells in S-phase. However, further delay occurs in the phase cell G2 / M, loss of ability to form shoots, and reduction of viable MCF-7 cells in the population. Under the influence of fullerenes in a subpopulation that survives for a long incubation increased the content of cells in S-phase, which is important to find ways to improve the effectiveness of anticancer drugs. Furthermore, increasing the number of cells in the suspension fraction and, consequently, the number of tumor spheroids. The mechanism of action of the C60 has not yet been elucidated. It can be implemented at the level of impact on the structure and function of plasma membrane and focal contact with the substrate, and by acting on the mechanisms of the regulatory signals by activating / inactivating cyclin-dependent kinases or other intracellular mediators of cell cycle progression. Therefore, in our opinion, fullerenes are a potential threat to the progression of the malignant process in the body. Single-wall carbon nanotubes, to our knowledge, is the most promising material for use as the extracellular matrix, as capable of forming self-assembled arrays of artificial substrate and to provide for the growth and proliferation of cells in suspension and the formation of cell aggregates up to 1 mm 3. In addition WHS have a positive Zeta potencial (to + 49.5mV), ensures that the electrostatic interaction with the negatively charged cell membrane (20,5mV). Simultaneously, the ability to create conditions for VHV growth of cells in suspension is a potential threat as well as fullerenes, for the development of cancer. Thus, as a result of operation, it was found that the apparent presence in the culture medium reduces the carbon nanostructures cell adhesion to the culture substrate and stimulates production of a large number of cell spheroids. Due to the modification of the physico-chemical properties, we used nanomaterials may influence the biology of cells depending on the tasks of biotechnology. Thus, it is possible to receive the directed growth of the cell population and cell spheroids large volume. Or, conversely, stimulate apoptotic processes, reducing the survival of the cell population. Keywords: tumor cells of breast cancer, ultra dispersed nanodiamond multicellular spheroids. Product Description popup.authors Бурлака Д.П. Гергелюк Т.С. Дем'яненко Д.П. Перепелиціна О.М. Рудь О.Д. Юрченко Н.П. Якимчук О.М. Ястребова О.В. popup.nrat_date 2020-04-02 Close