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Information × Registration Number 0215U004892, 0113U003847 , R & D reports Title Improve methods of reproduction and cultivation of valuable subjects in pond and industrial aquaculture popup.stage_title Head Sergiy Melnychuk, Registration Date 25-01-2016 Organization National University of Life and Environmental Sciences of Ukraine, Ukrainian Laboratory of Quality and Safety of Agricultural Products popup.description2 The object of study is the technology of artificial reproduction of valuable representatives of freshwater aquaculture. The aim of the study is to improving technology for posterity facilities pond and industrial fish farming and the development of methods for the control of fish floor. Theoretical and methodological basis of the study are the common in fisheries science, histological techniques, hydro-biological, biochemical, fish and molecular genetic studies. The value of these results is to improve the method of induction gynogenesis sturgeon based on physical methods of influence on sexual products. As a result of this work was to determine the optimum exposure settings sperm Bester ultraviolet rays at a dose of 200 J / m 2 and the induction gynogenesis for fertilized eggs starlet heat shock at 34 ° C. The origin of the resulting offspring ginogenetichnogo confirmed by examination of microsatellite DNA markers. The study results confirm the possibility of using this approach to create a stock selection and breeding core manufacturers, which will consist only of females to get them food caviar and improve the efficiency of fish reproductive systems. In addition, the condition will be reached preserve valuable and endangered aquaculture due to their more efficient use in artificial reproduction and cultivation at the present stage of increased anthropogenic impact on natural ecosystems. It has been found for the conditions of artificial repdoduction that drugs that cause secretion of gonadotropins in pituitary body of fish are not inferior to the drug derived from the fish pituitary. They are also convenient to use, have a mild effect, and do not cause blood clots in the oviducts of females. It has also been found for female starlet that are kept in controlled water temperature conditions that to prevent disruption of oogenesis in fish gonads it is necessary to create an artificial wintering season with water temperature below 6 degrees C before the spawning period. General anesthetic effect of the clove oil emulsion on silver carp and sterlet has also been studied. We developed the dosage for each fish species, depending on the sex and temperature of water where the fish is kept. We also tested the degumming effectiveness of 'tannin' on sterlet caviar. Effective drug concentration in aqueous solution was increased, compared to the one recommended by the scientific and technical sources. We have also found occurrences of secondary adhesiveness in eggs in 4-5 minutes after their artificial insemination and we developed a technique to eliminate the negative effects of this phenomenon. The use of these approaches are possible in the area of the aquaculture of Ukraine.54815481 Product Description popup.authors Коваленко Василь Олександрович Курта Христина Миколаївна Малишева Ольга Олексіївна Мельничук Сергій Дмитрович Поплавська Олена Сергіївна Спиридонов Владислав Геннадійович Шумова Віта Миколаївна popup.nrat_date 2020-04-02 Close