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Registration Number

0215U007055, 0110U006682 , R & D reports

Title

3.Role of nitric oxide signaling in plant defence and regulation of cytoskeleton induced by UVB-light oxidative stress.

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Head

Lytvyn D.I.,

Registration Date

27-01-2015

Organization

Institute of Food Biotechnology and Cenomics of the National Academy of Sciences of Ukraine

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The conditions for the quantitative determination of oxidative stress (OS) level in a plant cell line using A. thaliana/GRX-roGFP were selected. It was investigated the influence of UV-B and nitric oxide donors on the development of OS in the cells of A. thaliana. It was shown that microtubules are molecular targets for UV-B and its effects are realized in the OS induction, changes the morphology of the main root and in reorganization of microtubules. Treatment of A. thaliana seedlings with NO donor, followed by irradiation with UV-B prevents depolymerization of microtubules, suggesting a possible functional relationship between the content of NO in the cells and stability of the cytoskeletal components. It was found that protective effect of NO donor sodium nitroprusside is in a concentration range of 10-100 uM, higher concentrations have the opposite effect and cause the development of OS, cytoplasm acidification and development of programmed cell death

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Ємець А.І.
Ожерєдов С.П.
Танасієнко І.В.

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2020-04-02