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Registration Number

0215U007101, 0113U002585 , R & D reports

Title

Investigation of phospholipid content, activity and expression of Ca2+-ATPase genes with the help of cytochemistry, biochemystry, molecular biology and inhibitor analysis (blochers of Ca2+-sensitive channels) in normal conditions and under slow horizontal clinorotation.

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Head

Kordyum Yelizaveta Lvovna,

Registration Date

29-01-2015

Organization

M.G. Kholodny Institute of Botany NASU

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The data of the first point of the work on gravisensitivity of the cytoplasmic membrane lipid bilayer have been confirmed based on the changes in the content of separate phospholipids, saturated and unsaturated fatty acids in the microsomal fraction from pea seedling roots under clinorotation in comparison with the control. It was shown that a new balance, which is established between increasing and decreasing of saturated and unsaturated fatty acids under clinorotation, provides the similarity of an nonsaturation index (a ratio of unsaturated/ saturated fatty acids) with that in the control, that maintains cytoplasmic membrane fluidity (microviscosity) in the stationary limits and it is considered as an adaptation sign. The similarity of the cytoplasmic membrane fluidity under clinorotation and in the control, that was demonstrated by liquid chromatography, has been confirmed by the results of an analysis of spin probe EPR spectra which had an identical character in the microsomal fraction in the control and under clinorotation. It was shown that Са2+-ATPase activity localization in cells of the root distal elongation zone of pea seedlings under clinorotation differed from that in the control. As this enzyme plays a key role in the maintenance of calcium homeostasis in a cell, a decrease in the Са2+-ATPase activity may lead to the calcium concentration increasing in the cytosol under clinorotation.

Product Description

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Артеменко Ольга Анатоліївна
Жупанов Іван Вікторович
Заславський Віктор Абрамович
Кордюм Єлизавета Львівна
Недуха Олена Макарівна
Овруцька Ірина Іридіївна

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2020-04-02