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Information × Registration Number 0215U007126, 0113U002152 , R & D reports Title To develop medicophysical bases of magnetic nanotherapy technology for malignant neoplasms popup.stage_title Head Schepotin I.B, Registration Date 13-02-2015 Organization National Cancer Institute popup.description2 Object of research - magnetic nanocomplex consisting of micro- and nanoparticles Fe3O4 or Fe2O3 and doxorubicin; localizers, cell lines, T47D - human breast cancer; HeLa S3 - cervical cancer; A-549 - human lung cancer; tumor-bearing animals - carcinosarcoma Walker-256 and Guerin sarcoma. Equipment - mechanomagnetochemical reactor; apparatus for local inductothermy "Magniterm" (Radmir, Ukraine); mercury thermometer S195 (Medisana AG, Hilden, Germany); 300 mm ruler. Purpose - to develop medical and physical basis of magnetic nanotherapy based on the effect of the joint action of the inhomogeneous permanent magnetic and radiofrequency hyperthermia, and magnetic nanocomplex in experimental models of cancer process. Research methods - physical and technical, physical and chemical, immuno-cytochemical, laboratory and statistical. It is shown that the magnetic properties and antitumor effect of magnetic nanocomplex with doxorubicin and Fe3O4 nanoparticles on experimental animals with carcinosarcoma Walker-256 was higher than nanocomplex with Fe2O3 nanoparticles and officinal doxorubicin. Was designed and manufactured special stand for modeling distribution of nanoparticles in the liquid under the influence of the permanent magnetic and electromagnetic fields using three types of different localizers with different shapes and designs. Conducted studies using this stand in physiological solution showed that the area and the spatial distribution of the nanoparticles depend on the magnetic field lines created by localizers of different designs. Use in combination treatment of animals with carcinosarcoma Walker-256 and Guerin carcinoma magnetic dipole applicator with pin locator and magnetic nanocomplex with Fe3O4 nanoparticles and doxorubicin leads to inhibition of tumor growth, respectively, in 2.9 times and on 28% higher than in the treatment of officinal doxorubicin and electromagnetic radiation. Investigation of mechanisms of the antitumor effect of magnetic nanotherapy technology on the T47D, HeLa S3 and A-549 cell cultures on the basis of the study of immunophenotype proteins p21WAF1, betta-catenin and topoisomerase II alpha revealed that magnetochemical factor initiated by the magneto-dipole applicator with a pin and a magnetic localizer and nanocomplex causes relocalization of some antigens depending on the cell type. Field of application - oncology. Product Description popup.authors Бездєнєжних Наталія Олександрівна Дзятковська І.І. Дзятковська Наталія Миколаївна Крючков Євген Іванович Ніколов Микола Олександрович Орел Валерій Еммануїлович Рихальський Олександр Юрійович Романов Андрій Вікторович popup.nrat_date 2020-04-02 Close