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Information × Registration Number 0215U007161, 0114U002846 , R & D reports Title t o study the risk of development of prognostic unfavorable genetic lesions in chronic lymphocytic leukemia patients sufferers due to Chornobyl NPP accident depend on p53-associated apoptosis gene polymorphisms popup.stage_title Head Chumak Anatoliy Andriyovych, Доктор медичних наук Registration Date 16-02-2015 Organization State institution "National research centеr for radiation medicine of the National academy of medical sciences of Ukraine " popup.description2 The aim of work - to study the significance of gene polymorphisms in p53-depended apoptosis (TP53 codon 72 Arg/Pro, p21 codon 31 Ser/Arg, MDM2 SNP309) for the risk of chronic lymphocytic leukemia development and its behaviour in sufferers of Chornobyl NPP catastrophe. The object of study: 320 patients with chronic lymphocytic leukemia (CLL), including 107 ionizing radiation (IR) exposed patients due to Chornobyl NPP catastrophe, and 145 individuals without oncohematological pathology (73 sufferers of the Chornobyl NPP catastrophe and 72 IR-non-exposed persons). Methods: the determination of ТР53, MDM2, р21 gene's polymorphism by polymerase chain reaction and restriction of products, clinical, statistical. The main results: the distributions of polymorphisms rs6449182 of CD38 gene, rs966221 of PDE4D gene, and rs2430561 of interferon-gamma gene in the control group did not differ depend on radiation anamnesis of persons and corresponded to the others groups of Caucasians. No differences were revealed among IR-exposed and IR non-exposed CLL patients, except higher frequency of polymorphic allele of р21 gene among IR-exposed CLL parients compared wirh IR non-exposed ones (р=0.033), first of all in combination with genotype Arg/Pro of rs1042522 ТР53 gene and genotype ТT of SNP309 MDM2 gene (р=0,022). It was established negative influence of genotype GG of SNP309 MDM2 gene's polymorphism: higher frequency of CLL patients on terminal stages at diagnosis according classification of Binet (р=0.057) or Rai (р=0.028); shorter period of disease progression (р=0,019) and development of autoimmune hemolytic anemia (р=0,038); shorter median of overall survival in patients with unmutated IGHV genes (р=0.005). Elevated risk of Richter transformation in CLL patients, carriers of heterozygote genotype of rs1042522 ТР53 gene's polymorphism: OR = 3.029 (95% СІ 1.17 - 7.54; р=0.017), and increased frequency of haplotype Arg/Pro ТР53/Ser/Ser р21/ТG MDM2 in patients with Richter transformation (р=0.001) were revealed. Product Description popup.authors Абраменко Ірина Вікторівна Білоус Надія Іванівна Дягіль Ірина Сергіївна Плескач Гліб Вадимович popup.nrat_date 2020-04-02 Close