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Registration Number

0215U008534, 0115U001380 , R & D reports

Title

Creation of a molecular system for delivery of specific proteins in tumor cells based on the interaction of gold nanoparticles with proteins of different classes

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Head

Kashuba Olena Vitaliivna, Доктор біологічних наук

Registration Date

28-12-2015

Organization

R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology

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Discovered a new regulatory mechanism stem and differentiation of cells, depending on the protein overe[pression S18-2. Therefore, it is important to develop new methods for delivering proteins that regulate the cell cycle and stem cell/cell differentiation as an example S18-2 and RB proteins. To create new delivery systems polypeptides in a cell on nanoparticles as carriers in the first phase peptides were synthesized nanoparticles of gold and silver of different size (30-80 nm). Gold nanoparticles were synthesized on restoration aurata NaBH4 at room temperature. Silver nanoparticles was obtained in the recovery of silver nitrate solution using ammonia water. Retrieved purified GST-fusion proteins S18-2 and RB. These proteins and mutant RB proteins and pure GST-protein was induced in bacterial cultures (BL21 PLys) using IPTG.

Product Description

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Бучинська Любов Георгіївна
Кашуба Олена Віталіївна
Ковалевська Лариса Миколаївна

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2020-04-02