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Registration Number

0215U008572, 0115U004171 , R & D reports

Title

Studying on plant stress adaptation mechanisms and creation of resistant lines using experimental transgenesis protocols

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Head

Sheludko Yuriy Vsevolodovich,

Registration Date

29-12-2015

Organization

Institute of cell biology and genetic engineering NAS of Ukraine

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Synthetic promoters were constructed for cold-inducible transgene expression in plants. There are the core element of the cauliflower mosaic virus 35S promoter and either the DRE element from Arabidopsis thaliana COR15a gene or the ICE one from DREB1B gene promoter. The transription features of bifunctional reporter GFP-lichenase gene in plants of Nicotiana excelsior were investigated by transient expression method. It was shown that under temperature reduction the 35S-DRE promoter proved significantly higher expression of reporter gene in comparison to both the control one bearing the core element of the 35S promoter and synthetic 35S-ICE promoter. Potato and winter canola cultivars were selected for future experiments for stable genetic transformation. The transformation protocols were proposed.

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Герасименко Ірина Михайлівна
Кирпа-Несміян Тетяна Миколаївна
Рудас Володимир Андрійович
Сахно Людмила Олександрівна

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2020-04-02